rosettesep human naive cd4 t (cd45ro- ) cell enrichment kits Search Results


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STEMCELL Technologies Inc rosettesep human cd4+ t cell enrichment cocktail
Rosettesep Human Cd4+ T Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rosettesep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Easysep Human Naive Cd4 + T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rosettesep Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells ([%CD45RA- <t>CD45RO+</t> CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.
Cd45ro (Uchl1), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cd45ra (hi100)
PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells <t>([%CD45RA-</t> CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.
Cd45ra (Hi100), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc rosettesep cd8 + t cell enrichment mixture
PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells <t>([%CD45RA-</t> CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.
Rosettesep Cd8 + T Cell Enrichment Mixture, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson facsaria flow cytometer
PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells <t>([%CD45RA-</t> CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.
Facsaria Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson phycoerythrin-cy5-conjugated anti-cd3
PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells <t>([%CD45RA-</t> CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.
Phycoerythrin Cy5 Conjugated Anti Cd3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells ([%CD45RA- CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.

Journal: Journal of Immunology (Baltimore, Md. : 1950)

Article Title: Pre-B cell leukemia homeobox 1 is associated with lupus susceptibility in mice and humans

doi: 10.4049/jimmunol.1002362

Figure Lengend Snippet: PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells ([%CD45RA- CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.

Article Snippet: Human CD4 + T cells were enriched from peripheral blood with the RosetteSep kit (StemCell Tech.) and stained with CD3 (SK7), CD4 (RPA-T4), CD45RA (HI100), CD45RO (UCHL1), or isotype controls. mAbs were purchased from either BD Biosciences or eBioscience.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY

PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells ([%CD45RA- CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.

Journal: Journal of Immunology (Baltimore, Md. : 1950)

Article Title: Pre-B cell leukemia homeobox 1 is associated with lupus susceptibility in mice and humans

doi: 10.4049/jimmunol.1002362

Figure Lengend Snippet: PBX1-d expression is more frequent in lupus patients and correlates with an increased percentage of central memory CD4+ T cells. A. RT-PCR displaying the PBX1-a and PBX1-d isoforms in CD4+ T cells from three representative patients. B. PBX1 isoform distribution in 58 HC and 95 SLE patient samples. The percentage of samples expressing PBX1-a is represented in black, PBX1-d in white, and the co-expression of both by a hatched pattern. C. Comparison of the presence (grey) or absence (black) of PBX1-d expression between samples from patients that were treated or non-treated with steroids D. PBX1 isoform distribution in samples from HCs and SLE patients non-treated with steroids. Percentage of CD3+ CD4+ in HC and SLE patient PBLs (E) and in the total cohort according to the PBX1 isoform expression (F). Relative levels of memory CD4+ T cells ([%CD45RA- CD45RO+ CD4+ CD3+] - [%CD45RA+ CD45RO- CD4+ CD3+]) / [%CD45RA- CD45RO+ CD4+ CD3+] in HC and SLE patient PBLs (G), and in the total cohort according to the PBX1 isoform expression (H). Naïve CD45RO- CD62L+ to central memory CD45RO+ CD62L+ CD4+ T cells ratios in HC and SLE patient PBLs (I), and in the total cohort according to the PBX1 isoform expression (J). B-D: p values correspond to chi-square tests. E-J: Medians and interquantile ranges, p values correspond to Mann-Whitney tests; *: p ≤ 0.05, ** : p ≤ 0.01.

Article Snippet: Human CD4 + T cells were enriched from peripheral blood with the RosetteSep kit (StemCell Tech.) and stained with CD3 (SK7), CD4 (RPA-T4), CD45RA (HI100), CD45RO (UCHL1), or isotype controls. mAbs were purchased from either BD Biosciences or eBioscience.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY